tag:blogger.com,1999:blog-4387100009341358284.post6127166190086002750..comments2024-03-12T09:55:15.923+01:00Comments on Curly Arrow: Let's talk about TLCs Part 2 - Hanessian's StainDaniel Sejerhttp://www.blogger.com/profile/12498255536497699232noreply@blogger.comBlogger19125tag:blogger.com,1999:blog-4387100009341358284.post-81079968335449533432021-01-11T22:50:34.984+01:002021-01-11T22:50:34.984+01:00Nihydrin can form imine , 2,4-dnp can form imine,....Nihydrin can form imine , 2,4-dnp can form imine,.....then, what kind of chemical reaction can expert with this CAM TLC strain?Singireddihttps://www.blogger.com/profile/14905807561872958135noreply@blogger.comtag:blogger.com,1999:blog-4387100009341358284.post-4121330753165057782011-10-08T21:42:42.569+02:002011-10-08T21:42:42.569+02:00Can anybody explain the mechanism involved in this...Can anybody explain the mechanism involved in this stain?Vikramhttps://www.blogger.com/profile/04383522155141813115noreply@blogger.comtag:blogger.com,1999:blog-4387100009341358284.post-65489041719079204582009-08-26T14:41:17.856+02:002009-08-26T14:41:17.856+02:00Hmm, we don't store it in the fridge, so that ...Hmm, we don't store it in the fridge, so that might be it :D.Unknownhttps://www.blogger.com/profile/10466022823381810716noreply@blogger.comtag:blogger.com,1999:blog-4387100009341358284.post-53926187971185109992009-08-26T14:35:57.905+02:002009-08-26T14:35:57.905+02:00Morridin, I don't know. I've never had any...Morridin, I don't know. I've never had any problems with the stability of PMA or CAM. I normally store it in the fridge so it lasts a long, long time. D!Daniel Sejerhttps://www.blogger.com/profile/12498255536497699232noreply@blogger.comtag:blogger.com,1999:blog-4387100009341358284.post-61564335176035587222009-08-26T14:32:56.934+02:002009-08-26T14:32:56.934+02:00Thanks, and what about the light sensitivity and g...Thanks, and what about the light sensitivity and general stability of the CAM reagent? Is it better than PMA?Unknownhttps://www.blogger.com/profile/10466022823381810716noreply@blogger.comtag:blogger.com,1999:blog-4387100009341358284.post-65933417970775187542009-08-26T14:10:45.968+02:002009-08-26T14:10:45.968+02:00Morridin, spots appear within a minute with a powe...Morridin, spots appear within a minute with a powerful heatgun. Spots do fade realtively fast but different spots fade at different rates. I usually photocopy my plates the same day that I have developed them. D!Daniel Sejerhttps://www.blogger.com/profile/12498255536497699232noreply@blogger.comtag:blogger.com,1999:blog-4387100009341358284.post-59013969538482737302009-08-26T14:06:44.864+02:002009-08-26T14:06:44.864+02:00Ok, so it probably takes a few minutes only for th...Ok, so it probably takes a few minutes only for the spots to appear. I forgot to ask - as was mentioned PMA is quite light-sensitive reagent - is it the same with CAM, or it is more stable? And thanks for the quick answer :)Unknownhttps://www.blogger.com/profile/10466022823381810716noreply@blogger.comtag:blogger.com,1999:blog-4387100009341358284.post-263190293375773952009-08-26T13:58:55.548+02:002009-08-26T13:58:55.548+02:00Morridin, standard lab heatgun at maximum heating ...Morridin, standard lab heatgun at maximum heating until spots appear on the plate. The stain is water based so it needs a fair bit of heating. D!Daniel Sejerhttps://www.blogger.com/profile/12498255536497699232noreply@blogger.comtag:blogger.com,1999:blog-4387100009341358284.post-20972300486082859222009-08-26T13:56:23.068+02:002009-08-26T13:56:23.068+02:00Hi,
At our lab we have been using PAM for a few y...Hi,<br /><br />At our lab we have been using PAM for a few years now, but lately when trying to apply quantitative measuring procedures problems came up; so i ended up here today, thinking about moving to CAM. My question is about the heating - what do you use for heating? what temperature and for how long do you heat it? I would greatly appreciate your help :)Unknownhttps://www.blogger.com/profile/10466022823381810716noreply@blogger.comtag:blogger.com,1999:blog-4387100009341358284.post-7267645834742656322008-04-29T01:41:00.000+02:002008-04-29T01:41:00.000+02:00I would have to disagree regarding vanilin stains ...I would have to disagree regarding vanilin stains capabilities when compared to Hanessian stain. Vanilin is my preferred stain (see post at Curly Arrow by following the TLC label) because it is easy to handle and it colours different types of compounds differently. However, Hannessian stain is significantly more sensitive. I have had many compounds that didn't show up with vanilin at all but are Daniel Sejerhttps://www.blogger.com/profile/12498255536497699232noreply@blogger.comtag:blogger.com,1999:blog-4387100009341358284.post-73339860465531236702008-04-28T20:01:00.000+02:002008-04-28T20:01:00.000+02:00Well, another good recipe is the Vanillin-one as i...Well, another good recipe is the Vanillin-one as it stains almost the same things like CAM and gives different colours as well!Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-4387100009341358284.post-42577145466752206162007-01-22T15:30:00.000+01:002007-01-22T15:30:00.000+01:00Thanks for the stain recipe...i have heard of it, ...Thanks for the stain recipe...i have heard of it, but never tracked down the recipe. thanks again.Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-4387100009341358284.post-11548582221220446642006-12-04T02:15:00.000+01:002006-12-04T02:15:00.000+01:00thomas...
it certainly is nice when products have...thomas...<br /><br />it certainly is nice when products have a characteristic color on TLC. I make compounds that very commonly turn red after sitting on the TLC plate in air for an hour or so (they have sulfur in them). This has often been more indicative than the crude NMR data.Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-4387100009341358284.post-16571432179067787342006-12-01T00:35:00.000+01:002006-12-01T00:35:00.000+01:00The thing I like about anisaldehyde is that often ...The thing I like about anisaldehyde is that often times analogs in a series will stain with very similar color. In a past project it made quick work of finding my product (stained a characteristic blue color)amongst a myriad of other stuff.Thomashttps://www.blogger.com/profile/02939450039030689367noreply@blogger.comtag:blogger.com,1999:blog-4387100009341358284.post-12041245210273315682006-11-29T00:02:00.000+01:002006-11-29T00:02:00.000+01:00This is the best general stain there is. I have ru...This is the best general stain there is. I have run into compounds that don't work with phosphomolybdic acid and CAM has always saved my bacon!(mmmm...baaaacon...gggrrrggggg gggrrrrgggg) This is my first choice stain.Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-4387100009341358284.post-6358131942127193482006-11-28T23:48:00.000+01:002006-11-28T23:48:00.000+01:00Oooops a bit of an inconsistency between formulas ...Oooops a bit of an inconsistency between formulas and names in the recipe. Should all be OK now. There are numerous variations on this recipe. It doesn't seem to make any difference if you use Cerium Ammonium Nitrate / Sulfate / etc. As far as I know it all works fine so it's basically down to availablility and price. I've corrected the previous recipe and added another version of the stain. I Daniel Sejerhttps://www.blogger.com/profile/12498255536497699232noreply@blogger.comtag:blogger.com,1999:blog-4387100009341358284.post-56668927965865034172006-11-28T20:09:00.000+01:002006-11-28T20:09:00.000+01:00Is that Hanessian as in Stephen Hanessian at Unive...Is that Hanessian as in Stephen Hanessian at Universite de Montreal? We always call this stuff CAM.Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-4387100009341358284.post-56726838024043634742006-11-28T17:10:00.000+01:002006-11-28T17:10:00.000+01:00Arrhhhh.... so that is what we have in this jar la...Arrhhhh.... so that is what we have in this jar labelled "cerium". Excellent stain indeed!Christian Bechhttps://www.blogger.com/profile/07268699468331519982noreply@blogger.comtag:blogger.com,1999:blog-4387100009341358284.post-79284329204914383952006-11-28T15:34:00.000+01:002006-11-28T15:34:00.000+01:00yeah, that's my usual stain...stains almost anythi...yeah, that's my usual stain...stains almost anything, but particularly alcohols and silyl ethers well. then...anisaldehyde and permanganate...Anonymousnoreply@blogger.com